Leica sp2 confocal manual

Leica SP8 TCS Users Manual Follow the procedure for start up and log on as posted in the lab. Please log on with your account only and do For true confocal microscopy, you always want to choose Airy 1. When you hit Airy 1, the red slider will adjust the pinhole to the appropriate. Leica SP5 Inverted Confocal Manual. Capabilities: Imaging diverse fluorophores in fixed or live samples. Temperature, humidity, CO2 control. Spectrally tunable detectors. Ultra-fast resonant scanning (reduced photo-damage with live samples) 3D z-stacks, timelapse, multi-position timelapse. FRAP/FLIP/Photoactivation, FRET (ratio or acceptor. All rights to this document are held by Leica Microsystems CMS GmbH. Adaptation, translation and reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express .

TAMU MIC Leica SP8 confocal/STED/FLIM user guide Last updated Desc-1st You must read the MIC Facility Manual before training and satisfy the BSL-2 training requirements (outlined in a separate document from the instrumewnt web page). The MIC facility manual covers lab and laser safety, training policy, scheduling, and biosafety requirements. Page 8 User Manual TCS SP5 V: 02 | Document-ID-No.: REVISIONSANDCHANGES Leica Microsystems CMS GmbH reserves the right to revise this document and/or to further develop and improve the products described in this document at any time wi-thout prior notice or any other obligation. Information and specifications in this manual. Page 1 LMW Microscopy Lab: Leica SP2 Page 1 `6/26/ Manual for Leica SP2 Confocal Microscope Enter you name, the date, the time, and the account number in the user log book. Things to check before start‐up. Make sure that your sample slides are clean and sealed. Use Windex and cotton balls or Kimwipes to clean coverslips.

o Use the first option if you need confocal only. o Use the second option for confocal and Multiphoton imaging with HYD-RLD plus 2TLD. o Use the third option for confocal and Multiphoton imaging with HYD-RLD - If your project requires, you can turn ON “Resonant scanner” at this point. Otherwise you can leave it “off”. Leica SP5 Inverted Confocal Manual. Capabilities: Imaging diverse fluorophores in fixed or live samples. Temperature, humidity, CO2 control. Spectrally tunable detectors. Ultra-fast resonant scanning (reduced photo-damage with live samples) 3D z-stacks, timelapse, multi-position timelapse. FRAP/FLIP/Photoactivation, FRET (ratio or acceptor. Manual for Leica SP2 Confocal Microscope. Enter you name, the date, the time, and the account number in the user log book. Things to check before start-up. • Make sure that your sample slides are clean and sealed. Use Windex and cotton balls or Kimwipes to clean coverslips. Fixed samples need to be sealed with nail polish. • Check the objectives.