Invitrogen purelink rna mini kit manual

Types of kits This manual supports the following kit. Kit Reactions Cat. No. PureLink™ Viral RNA/DNA Mini Kit 50 Contents The components and amounts included in the PureLink ™ Viral RNA/DNA Mini Kit are listed in the following table. Note: Since the kit is File Size: KB. The Invitrogen PureLink RNA Mini Kit provides rapid column based purification of total RNA from a wide range of cell and tissue types. High-quality RNA in less than 20 minutes. Up to 1, ug of purified RNA from a single extraction. No hazardous organic (phenol/chloroform) extraction lysis. the Kit The PureLink® RNA Mini Kit offers the following advantages: • RNA isolation from a wide variety of sample types and amounts • Minimal genomic DNA contamination of the purified RNA and an optional on-column DNase digestion • Rapid and convenient column purification proceduresFile Size: KB.

PureLink™ Viral RNA/DNA Mini Kit From Invitrogen. The Good. Kit is easy to use. Allows for use of up to ul of the starting materials with elution of the final viral nucleic acids in as low as 10 ul; thus allows enrichment of viral RNA/DNA for sensitive downstream analysis. The Bad. Experienced Users' Mini Kit Procedure Introduction This quick reference sheet is included for experienced users of the PureLink™ Genomic DNA Mini Kit. For more details, refer to this manual. Step Procedure Preparing lysates Prepare the lysate using an appropriate sample preparation protocol as follows: Sample Amount Page no. The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris.

The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination. Simplified, nontoxic RNA purification. long-term storage, keep the purifi ed RNA at –80°C. Perform DNase I treatment after purifi cation (refer to the PureLink® RNA Mini Kit manual) to assure highly pure RNA without genomic DNA contamination. Determine the quality and quantity of your RNA by UV absorbance at nm. Page 3 RNA Storage Binding, Washing, and Elution of RNA. PureLink™ Viral RNA/DNA Mini Kit From Invitrogen. There are several different viral RNA (vRNA) kits available for vRNA extraction. For example, the EZ1 Virus Mini Kit from Qiagen, working together with the BioRobot EZ1 workstation, is a very neat system and will totally free you from manual extraction. However, the maximum amount of starting plasma for the machine is ul, and the minimum elution volume for vRNA is 60 ul.